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Effects of Lalistat 1 and bafilomycin A1 <t>(Baf-A1)</t> on cellular levels of CoQ. A , CoQ levels were increased after Lalistat 1 treatment. B , changes in CoQ levels after treatment with Baf-A1. Data were expressed as percentages relative to no-treatment controls set to 100%. Bars represent means ± SEM (n = 3–4 per group). ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001 vs. non-treatment controls; ## p < 0.01 when the indicated groups are compared (one-way ANOVA followed by Sidak's post hoc comparison test). C , Western blot determination of LC3 protein levels. A representative image is shown. Band density ratios of LC3-II normalized to the loading control GAPDH are reported as mean ± SEM. ∗p < 0.05 compared to the no-treatment control. Iron or NAC treatment did not increase the LC3-II levels, nor did it affect the increased conversion of LC3-I to LC3-II due to Baf-A1 co-treatment (one-way ANOVA followed by Sidak's post hoc test).
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Effects of Lalistat 1 and bafilomycin A1 <t>(Baf-A1)</t> on cellular levels of CoQ. A , CoQ levels were increased after Lalistat 1 treatment. B , changes in CoQ levels after treatment with Baf-A1. Data were expressed as percentages relative to no-treatment controls set to 100%. Bars represent means ± SEM (n = 3–4 per group). ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001 vs. non-treatment controls; ## p < 0.01 when the indicated groups are compared (one-way ANOVA followed by Sidak's post hoc comparison test). C , Western blot determination of LC3 protein levels. A representative image is shown. Band density ratios of LC3-II normalized to the loading control GAPDH are reported as mean ± SEM. ∗p < 0.05 compared to the no-treatment control. Iron or NAC treatment did not increase the LC3-II levels, nor did it affect the increased conversion of LC3-I to LC3-II due to Baf-A1 co-treatment (one-way ANOVA followed by Sidak's post hoc test).
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Effects of Lalistat 1 and bafilomycin A1 (Baf-A1) on cellular levels of CoQ. A , CoQ levels were increased after Lalistat 1 treatment. B , changes in CoQ levels after treatment with Baf-A1. Data were expressed as percentages relative to no-treatment controls set to 100%. Bars represent means ± SEM (n = 3–4 per group). ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001 vs. non-treatment controls; ## p < 0.01 when the indicated groups are compared (one-way ANOVA followed by Sidak's post hoc comparison test). C , Western blot determination of LC3 protein levels. A representative image is shown. Band density ratios of LC3-II normalized to the loading control GAPDH are reported as mean ± SEM. ∗p < 0.05 compared to the no-treatment control. Iron or NAC treatment did not increase the LC3-II levels, nor did it affect the increased conversion of LC3-I to LC3-II due to Baf-A1 co-treatment (one-way ANOVA followed by Sidak's post hoc test).

Journal: The Journal of Biological Chemistry

Article Title: Elevated cellular accumulation of endogenous and exogenous CoQ by altered intracellular trafficking

doi: 10.1016/j.jbc.2025.110878

Figure Lengend Snippet: Effects of Lalistat 1 and bafilomycin A1 (Baf-A1) on cellular levels of CoQ. A , CoQ levels were increased after Lalistat 1 treatment. B , changes in CoQ levels after treatment with Baf-A1. Data were expressed as percentages relative to no-treatment controls set to 100%. Bars represent means ± SEM (n = 3–4 per group). ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001 vs. non-treatment controls; ## p < 0.01 when the indicated groups are compared (one-way ANOVA followed by Sidak's post hoc comparison test). C , Western blot determination of LC3 protein levels. A representative image is shown. Band density ratios of LC3-II normalized to the loading control GAPDH are reported as mean ± SEM. ∗p < 0.05 compared to the no-treatment control. Iron or NAC treatment did not increase the LC3-II levels, nor did it affect the increased conversion of LC3-I to LC3-II due to Baf-A1 co-treatment (one-way ANOVA followed by Sidak's post hoc test).

Article Snippet: Lalistat one was purchased from Cedarlane Labs. Bafilomycin A1 (Baf-A1) was purchased from MedChemExpress.

Techniques: Comparison, Western Blot, Control